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Specimen Atlas of Research Peptides81 plates · MIT
Side-by-side · Research reference

ACE-031vsSurvodutide

Side-by-side comparison across mechanism, dosage, evidence, side effects, administration, and stack synergies. Citations on every claim where available.

APhase 2HUMAN-REVIEWED10/44 cited
BPhase 3HUMAN-REVIEWED25/54 cited
ACE-031
ActRIIB-Fc Fusion · Phase 2 Halted
Phase 2Highest trial stage
2011Development halted
~58.4 kDaMolecular weightReichel 2025
SQ · Weekly dosing investigated
Survodutide
GLP-1/Glucagon Dual Agonist · Phase 3
Once weeklyFrequency
Phase 3Development stageRubino 2026
GLP-1/GCGRDual targetZimmermann 2026
SQ · Once Weekly

01Mechanism of Action

Parameter
ACE-031
Survodutide
Primary target
Myostatin, GDF11, activin A — TGF-β superfamily ligands
GLP-1 receptor and glucagon receptor (GCGR)Yathindra 2026Zimmermann 2026
Pathway
Soluble decoy receptor binds circulating myostatin/TGF-β ligands → prevents ActRIIB activation → SMAD2/3 pathway inhibition
Central: CVOs → hypothalamic appetite regulation. Peripheral: GLP-1R → incretin effect; GCGR → hepatic lipid metabolism, energy expenditureZimmermann 2026Long 2026
Downstream effect
Disinhibition of myogenic signaling, increased skeletal muscle mass and strength
Decreased energy intake, increased energy expenditure, improved glucose homeostasis, hepatic fat reductionZimmermann 2026Yathindra 2026
Feedback intact?
Origin
Recombinant fusion protein: human ActRIIB extracellular domain + IgG1-Fc fragmentReichel 2025
Antibody development

02Dosage Protocols

Parameter
ACE-031
Survodutide
Clinical dosing
Weekly or biweekly SQ injections (exact doses undisclosed pre-halt)
Phase 2 DMD trial protocol not fully published.
Black market products
Variable purity; 12/14 tested products contained target protein plus contaminantsReichel 2025
SDS-PAGE revealed multiple protein bands; quality control absent.Reichel 2025
Evidence basis
Phase 2 trial discontinued — incomplete dataset
Phase 2 RCT (obesity) · Phase 3 ongoing
Half-life
Days to weeks (Fc-fusion typical kinetics)
IgG1-Fc domain confers extended circulation time.
Duration investigated
12–24 weeks (trial cut short)
Standard dose
Not yet disclosed (Phase 3 ongoing)
SYNCHRONIZE Phase 3 program underway.Rubino 2026
Frequency
Once weekly
Route
SubcutaneousYathindra 2026
Phase 2 findings
Significant weight loss and metabolic marker improvementYathindra 2026
MASH indication
Under investigation for MASH-cirrhosisPatil 2026Andonie 2026

03Metabolic / Fat Loss Evidence

Parameter
ACE-031
Survodutide
Primary fat target
Total body weight, visceral adipose tissue
Weight loss mechanism
Dual action: decreased energy intake + increased energy expenditureZimmermann 2026
Phase 2 efficacy
Significant weight loss demonstrated
Specific percentage not disclosed in abstracts.
Metabolic markers
Improvements in ALT, AST, LDL levels; significant ALT reduction (MD -22.10 vs placebo)Yathindra 2026Abulehia 2026Andonie 2026
MRI-PDFF reduction
Hepatic fat reduction demonstrated in MASH trialsAndonie 2026
Network meta-analysis
Favorable efficacy profile vs other glucagon receptor agonists
Hepatic requirement
Hepatic GCGR required for maximal weight loss and metabolic effectsLong 2026
Energy expenditure
Increased energy expenditure contributes to weight lossZimmermann 2026
Comparative efficacy
Network meta-analysis shows competitive efficacy in GRA class

04Side Effects & Safety

Parameter
ACE-031
Survodutide
Epistaxis (nosebleeds)
Significant incidence in Phase 2 DMD trial — primary safety signal
Telangiectasia
Dilated capillaries / spider veins observed
Vascular abnormalities
Mechanism: ActRIIB/ALK1 pathway disruption affects vascular homeostasis
Injection site reactions
Local erythema, induration (biologics class effect)
Expected with subcutaneous administration
Antibody development
Potential for anti-drug antibodies (Fc-fusion proteins); incidence not reported
Black market contaminants
12/14 tested products contained multiple unidentified proteins alongside ACE-031Reichel 2025
GI symptoms
Diarrhea, nausea, fatigue — class effect of GLP-1 agonists
Safety profile
Network meta-analysis: comparable safety to other GRAs
Serious adverse events
Monitored in Phase 2/3; no unique safety signals reported
Detailed SAE data pending Phase 3 completion.
Glucagon-related effects
Potential for tachycardia, increased blood pressure — theoretical glucagon effect
Absolute Contraindications
ACE-031
  • ·History of vascular disorders (epistaxis, telangiectasia, HHT)
  • ·Pregnancy (TGF-β pathway critical for fetal development)
  • ·Active malignancy (myostatin inhibition may affect tumour growth)
  • ·Use of non-pharmaceutical grade ACE-031 (contamination risk)Reichel 2025
Survodutide
  • ·Personal or family history of medullary thyroid carcinoma (class effect)
  • ·Multiple endocrine neoplasia syndrome type 2
Relative Contraindications
ACE-031
  • ·Coagulation disorders or anticoagulant use (epistaxis risk)
  • ·Hereditary hemorrhagic telangiectasia (HHT) family history
  • ·Cardiovascular disease (vascular remodeling effects unknown)
Survodutide
  • ·Severe GI disease (inflammatory bowel disease, gastroparesis)
  • ·History of pancreatitis
  • ·Cardiovascular disease (monitor closely for glucagon effects)

05Administration Protocol

Parameter
ACE-031
Survodutide
1. Pharmaceutical status
ACE-031 is not FDA-approved or commercially available. Phase 2 development was discontinued in 2011 due to safety concerns. Any ACE-031 on the black market is unregulated research chemical.
Specific reconstitution protocol not yet publicly disclosed. Follow manufacturer instructions upon approval.
2. Black market quality
12 of 14 tested black market ACE-031 products contained the target protein but also carried multiple unidentified protein contaminants detectable by SDS-PAGE. Two products contained no ACVR2B-immunoreactive material.Reichel 2025
Subcutaneous — abdomen, thigh, or upper arm. Rotate sites weekly to minimize injection site reactions.
3. Detection in sport
ACE-031 is prohibited under WADA S4.3 (Myostatin Inhibitors). Gel electrophoresis and Western blotting using ACVR2B-specific antibodies can detect the ~58.4 kDa protein in biological samples.Reichel 2025
Once weekly, same day each week. Can be administered at any time of day, with or without meals.
4. Clinical trial route
Phase 2 protocol used subcutaneous injections at weekly or biweekly intervals. Exact dosing protocols remain unpublished.
Store refrigerated (2–8 °C) until use. Do not freeze. Protect from light. Specific reconstituted storage duration pending labeling.
5. Needle
Subcutaneous injection with appropriate gauge needle (typically 27–31G). Use sterile technique.