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Specimen Atlas of Research Peptides81 plates · MIT
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XXIIPlate XXIIReviewed 2026-04-27

Follistatin-344

Myostatin Antagonist

also known as FST-344, Follistatin isoform 344

344-amino-acid isoform of follistatin, an endogenous glycoprotein that binds and neutralizes myostatin and activin A. Lacks the heparin-binding domain of FST-315, resulting in systemic circulation rather than tissue sequestration. Investigated as a myostatin antagonist for skeletal muscle hypertrophy and sarcopenia treatment. Mechanism centers on disruption of myostatin-ActRIIB signaling, relieving brake on muscle protein synthesis.

§ I

At a glance

FST/MSTN ratio ↑
15–25%
Circulating isoform
344 AA
Phase status
Research
Route

Research · No approved protocol

§ II

Mechanism

Edit ↗

Primary target — Myostatin (MSTN/GDF-8) and Activin A.

Pathway — FST-344 binds MSTN/Activin → prevents ActRIIB receptor engagement → disinhibits muscle anabolism.

Downstream effect — Elevated follistatin/myostatin ratio, increased muscle protein synthesis, attenuated muscle atrophy signaling [jeong-2026].

Origin — Endogenous glycoprotein, 344-AA isoform lacking heparin-binding domain (vs FST-315).

Feedback intact — Yes — indirect antagonist, preserves endogenous regulation.

§ III

Dosage

Protocols described in the cited literature; not medical advice.

Edit ↗
ParameterValue
Clinical protocolNone — no approved dosing regimenFollistatin-344 measured as endogenous biomarker, not administered exogenously in cited trials.
Research contextEndogenous modulation via exercise + nutritionResistance training + EAA intake elevated FST/MSTN ratio by 15–25% in 12-week RCT (older women).
Evidence basisHuman observational / biomarker studies
Half-lifeNot establishedCirculating isoform; lacks tissue-binding domain of FST-315.
§ III · b

Reconstitution

A pure mass-to-volume utility. Enter what you have in the vial; the atlas computes the volume per dose. No prescription information.

Inputs
mg
mL
mcg
The calculator does pure mass-to-volume math. It does not recommend a dose. Refer to Follistatin-344's cited literature for protocol specifics.
Volumetric outputFig. C — reconstitution math
Volume per dose
0.100mL
10.0 units on a U-100 insulin syringe
Concentration
2500
mcg per mL
Doses per vial
20
at this dose
§ IV

Evidence

Edit ↗
Strength
15/100
theoretical

No direct fat loss trials. Myostatin inhibitors focus on lean mass preservation; fat loss is indirect via muscle metabolic demand.

OutcomeFinding
Primary targetMuscle mass preservation, not direct lipolysis
Indirect fat effectIncreased lean mass → elevated resting metabolic rateNot primary mechanism. Muscle-sparing during deficit.
Clinical evidenceLorcaserin trial (6 mo) showed no MAFI axis changes during fat loss [ramirezcisneros-2026]Suggests follistatin not primary driver of fat loss in weight-reduction interventions.
GLP-1RA studiesLiraglutide (35 days) — no significant MAFI axis modulation despite fat/lean changes
§ V

Adverse events

Severities follow the FDA / CTCAE convention.

Edit ↗
Clinical safety datasevere
None — no human exogenous administration trials in literature
Theoretical risksmoderate
Excessive myostatin inhibition → muscle overgrowth, impaired glucose tolerance
Endogenous elevation (exercise)mild
No adverse effects reported in 12-week resistance + EAA trials
Cancer risk (theoretical)severe
Myostatin inhibition may promote tumor growth in malignancy (preclinical data)
Regulatory statussevere
Not approved for human use — research peptide only
Absolute contraindications
  • Active malignancy
  • No approved protocol — research use only
Relative contraindications
  • Insulin resistance / Type 2 diabetes (monitor glucose)
  • Pregnancy / lactation (unknown safety profile)
§ VI

Administration

Edit ↗
  1. 01
    Regulatory status

    Follistatin-344 is not approved for human administration. All cited studies measure endogenous serum follistatin as a biomarker, not as an exogenous therapeutic agent.

  2. 02
    Endogenous modulation

    Resistance exercise combined with essential amino acid (EAA) supplementation elevated the follistatin/myostatin ratio by 15–25% in 12-week randomized trials. Protein intake (1.2–1.5 g/kg/day) synergizes with training to upregulate endogenous follistatin.

  3. 03
    Measurement context

    Serum follistatin and follistatin/myostatin ratio are used diagnostically in sarcopenia screening and as biomarkers of muscle anabolic balance in clinical trials.

  4. 04
    Research consideration

    Gene therapy and recombinant follistatin delivery are under preclinical investigation for muscular dystrophy and sarcopenia. No human safety or efficacy data for exogenous FST-344 administration.

§ VII

Synergies

Edit ↗
multi-pathway synergy
Deterministic 12-node hex coat-of-arms fingerprint for Follistatin 344, generated from the slug hash. Decorative; the same slug always produces the same motif.+Deterministic 12-node hex coat-of-arms fingerprint for Bpc 157, generated from the slug hash. Decorative; the same slug always produces the same motif.
Follistatin-344 + BPC-157

Follistatin-344 (myostatin antagonist) and BPC-157 (tissue repair peptide) address complementary pathways in muscle recovery. FST-344 promotes muscle protein synthesis by disinhibiting myostatin signaling, while BPC-157 accelerates healing of tendons, ligaments, and microtears via angiogenesis and collagen synthesis. Combined, they may support both hypertrophy and structural repair during high-volume training or injury recovery.

Primary benefit — Muscle hypertrophy + accelerated soft tissue repair
moderate synergy
Deterministic 12-node hex coat-of-arms fingerprint for Follistatin 344, generated from the slug hash. Decorative; the same slug always produces the same motif.+Deterministic 12-node hex coat-of-arms fingerprint for Tb 500, generated from the slug hash. Decorative; the same slug always produces the same motif.
Follistatin-344 + TB-500

TB-500 (thymosin beta-4 fragment) promotes cell migration, angiogenesis, and anti-inflammatory signaling in muscle and connective tissue. Follistatin-344's anabolic signaling may synergize with TB-500's regenerative effects during muscle damage or overtraining, particularly in older adults where both myostatin inhibition and tissue repair are rate-limiting.

Primary benefit — Enhanced recovery, reduced inflammation, muscle growth support
Appendix

Sources

7%

of 58 rendered claims carry a resolvable citation.

  1. [boonpiraks-2026]
    Boonpiraks 2026Diagnostic Performance of Serum Adiponectin and a Biomarker-Based Model for Sarcopenia in Compensated Cirrhosis.
    journal, 2026
  2. [jeong-2026]
    Jeong 2026Effect of exercise on hormonal responses in adolescents with obesity and leptin resistance: a randomized trial.
    journal, 2026
  3. [ramirezcisneros-2026]
    Ramirez-Cisneros 2026Lorcaserin induces abdominal fat loss with associated improvements of the circulating metabolome/lipidome and no changes in the myostatin-activin-follistatin-IGF-1 axes: A 6-month long randomized placebo-controlled clinical trial.
    journal, 2026
  4. [samali-2025]
    Samali 2025Myostatin inhibitors in sarcopenia treatment: A comprehensive review of mechanisms, efficacy and future directions.
    journal, 2025
Plate composed 2026-04-27 · maturity human-reviewed · schema v1 · Contributors: peptidesdb-core · 54 fields uncited — open contributions