Kisspeptin-10
also known as KP-10, metastin 45-54, KISS1 C-terminal fragment
10-amino-acid C-terminal fragment of kisspeptin (KISS1 gene product). Activates GPR54/Kiss1R on hypothalamic GnRH neurons, triggering LH surge. Phase 1/2 trials in fertility research and hypothalamic amenorrhea. Core regulator of the hypothalamic-pituitary-gonadal axis, integrating metabolic and reproductive signals through direct GnRH neuron excitation.
At a glance
IV / SQ · Investigational
Primary target — GPR54/Kiss1R on hypothalamic GnRH neurons [rnnekleiv-2026][colladosole-2026].
Pathway — Kisspeptin → GPR54 activation → GnRH neuronal depolarization → Pulsatile GnRH release → Pituitary LH/FSH secretion [lages-2026][rnnekleiv-2026].
Downstream effect — Pulsatile LH surge, FSH elevation, gonadal steroidogenesis, gametogenesis initiation [lages-2026].
Origin — C-terminal decapeptide of KISS1 gene product; retains full biological activity of longer kisspeptin isoforms.
Feedback intact — Yes — integrates estradiol, leptin, and IGF-1 signals to modulate HPG axis [silva-2026][rnnekleiv-2026].
| Parameter | Value |
|---|---|
| Clinical trial dose | Phase 1/2 investigationalDosing protocols vary by indication (hypothalamic amenorrhea, IVF trigger). |
| Route | IV or SQ administrationIV preferred in controlled trials for precise pulsatile delivery. |
| Evidence basis | Phase 1/2 trials |
| Half-life | Short (minutes)Rapid clearance; pulsatile dosing mimics physiological GnRH pulse frequency. |
Reconstitution
A pure mass-to-volume utility. Enter what you have in the vial; the atlas computes the volume per dose. No prescription information.
- — Active pregnancy
- — Hormone-sensitive malignancy (breast, ovarian, endometrial)
- — Polycystic ovary syndrome (PCOS) without monitoring
- — Uncontrolled thyroid dysfunction
- 01Reconstitution (if lyophilized)
Reconstitute with sterile water or saline per protocol. Gently swirl — do not shake. Solution should be clear and colorless.
- 02Route selection
IV infusion for pulsatile delivery in clinical trials; SQ for outpatient protocols. IV allows precise temporal control of GnRH pulse frequency.
- 03Timing
Pulsatile dosing (e.g., every 60–90 min) mimics physiological GnRH pulse generator. Single-bolus protocols used for LH surge induction in fertility research.
- 04Monitoring
Serial LH, FSH, estradiol measurements to confirm HPG axis activation. Ultrasound monitoring for ovarian response in fertility applications.
- 05Storage
Lyophilized: store at 2–8 °C, light-protected. Reconstituted: refrigerate, use within 24–48 hours per protocol.
Sources
of 41 rendered claims carry a resolvable citation.
- [akhtar-2025]Akhtar 2025 — Molecular pathways affecting reproductive efficiency in seasonal breeders: prospects and implications for improving fertility in donkeys.
journal, 2025 - [colladosole-2026]Collado-Sole 2026 — Microglia Rank signaling regulates GnRH neuronal function and the hypothalamic-pituitary-gonadal axis.
journal, 2026 - [lages-2026]Lages 2026 — GH and GnRH-gonadotropin secretion.
journal, 2026 - [rnnekleiv-2026]Rønnekleiv 2026 — The role of hypothalamic kisspeptin neurons in coordinating reproduction and metabolism.
journal, 2026 - [silva-2026]Silva 2026 — KNDy neurons as an indirect target of insulin-like growth factor-1.
journal, 2026