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Specimen Atlas of Research Peptides85 plates · MIT
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C32H49N9O5 · 639.8 g/mol · PubChem CID 11764719

72Plate 72FDA approved · 2025Reviewed 2026-07-18

SS-31

Mitochondrial-targeted tetrapeptide

also known as Elamipretide, Bendavia, MTP-131

Synthetic four-amino-acid peptide that selectively localises to the inner mitochondrial membrane by binding cardiolipin. Stabilises cristae structure, preserves electron transport chain efficiency, and reduces ROS generation in stressed mitochondria. Other indications studied in Phase 2/3 trials — including ischemia-reperfusion and dry AMD — remain investigational, and research-grade SS-31 is not an approved product. Approval applies to the specific product Forzinity (elamipretide hydrochloride), granted US FDA accelerated approval on 19 September 2025 to improve muscle strength in adult and pediatric patients with Barth syndrome weighing at least 30 kg, with continued approval potentially contingent on confirmatory benefit. [fda-forzinity-label-2025]

Quick facts
Class
Mitochondrial-targeted tetrapeptide
Evidence level
FDA-approved
Regulatory status
FDA-approved (US)
§ I

At a glance

Daily dose
40 mg
Evidence level
FDA-approved
Half-life
~3 hr
Route

SQ · Abdomen · Once daily

§ II

Mechanism

Edit ↗

Primary target — Cardiolipin in inner mitochondrial membrane [szeto-2014].

Pathway — Cardiolipin binding → cristae stabilisation → ETC integrity → reduced ROS + preserved ATP synthesis [szeto-2014][szilagyi-2009].

Downstream effect — Mitochondrial bioenergetic preservation; cardio-, neuro-, and reno-protective effects in animal + clinical studies [szeto-2014].

Origin — Synthetic tetrapeptide D-Arg-Dmt-Lys-Phe-NH₂; cell-permeable, mitochondrial-selective [szeto-2014].

§ III

Dosage

Protocols described in the cited literature; not medical advice.

Edit ↗
ParameterValue
Standard dose40 mg / day SQ (clinical trials)Anecdotal community range 5-10 mg/day. Phase 3 trials use 40 mg.
FrequencyOnce daily
Lower / starter dose5 mg / day (anecdotal)
Evidence basisAccelerated approval (Forzinity) for Barth syndrome; AMD and ischemia-reperfusion remain investigational [fda-forzinity-label-2025]
DurationIndefinite for mitochondrial disease; cycled for healthspan use
ReconstitutionBacteriostatic water
TimingMorning fasted preferred; pre-workout for exercise-induced mitochondrial stress
Half-life~3 h plasma; tissue uptake longer
§ III · b

Reconstitution

A pure mass-to-volume utility. Enter what you have in the vial; the atlas computes the volume per dose. No prescription information.

Inputs
mg
mL
mcg
The calculator does pure mass-to-volume math. It does not recommend a dose. Refer to SS-31's cited literature for protocol specifics.
Volumetric outputFig. C — reconstitution math
Volume per dose
0.013mL
1.3 units on a U-100 insulin syringe
Concentration
20000
mcg per mL
Doses per vial
160
at this dose
§ V

Adverse events

Severities follow the FDA / CTCAE convention.

Edit ↗
Injection site reactionmild
Erythema, mild pruritus
GI symptomsmild
Nausea (uncommon)
Headachemild
Reported in some Phase 3 trials
Cardiovascularmild
Cardio-protective in studies; no signal of harm
Long-term safetymild
Phase 3 data over 24+ months; no major safety signals
Pregnancy / OBsevere
Avoid — insufficient data
Absolute contraindications
  • Pregnancy / breastfeeding
  • Hypersensitivity to peptide
Relative contraindications
  • None established
§ VI

Administration

Edit ↗
  1. 01
    Reconstitution

    Add bacteriostatic water per label. Light-protected handling.

  2. 02
    Injection site

    SQ — abdomen or thigh. Rotate sites.

  3. 03
    Timing

    Morning fasted; pre-workout for exercise-augmented mitochondrial stress.

  4. 04
    Storage

    Lyophilised: refrigerate, light-protected. Reconstituted: refrigerate ≤30 days.

  5. 05
    Needle

    29–31G, 4–8 mm insulin syringe.

§ VII

Synergies

Edit ↗
Reference Q&A

Frequently asked

How does SS-31 work?

SS-31 acts on Cardiolipin in inner mitochondrial membrane, signalling via Cardiolipin binding → cristae stabilisation → ETC integrity → reduced ROS + preserved ATP synthesis. [szeto-2014][szeto-2014][szilagyi-2009]

Is SS-31 FDA-approved?

Yes — SS-31 is approved by the US FDA.

Appendix

Sources

16%

of 43 rendered claims carry a resolvable citation.

  1. [fda-forzinity-label-2025]
    FORZINITY (elamipretide) injec 2025FORZINITY (elamipretide) injection prescribing information
    fda-label, 2025
    NDA 215244. Accelerated approval 2025-09-19 to improve muscle strength in adult and pediatric patients with Barth syndrome weighing at least 30 kg.
  2. [szeto-2014]
    Szeto 2014First-in-class cardiolipin-protective compound as a therapeutic agent to restore mitochondrial bioenergetics
    Br J Pharmacol, 2014
    SS-31 cardiolipin-binding mechanism review.
  3. [szilagyi-2009]
    Szilagyi 2009SS-31 protects mitochondria during ischemia-reperfusion
    Stroke, 2009
    Foundational SS-31 (Bendavia/Elamipretide) cardio-protective mechanism paper.
Related plates
Plate composed 2026-07-18 · maturity human-reviewed · schema v1 · Contributors: peptidesdb-core · 36 fields uncited — open contributions