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Specimen Atlas of Research Peptides81 plates · MIT
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67Plate 67Reviewed 2026-04-27

SNAP-8

SNARE Inhibitor Peptide

also known as Acetyl Octapeptide-3, Acetyl Glutamyl Heptapeptide-1, SNAP-8 peptide

Synthetic 8-amino-acid peptide derived from the N-terminal region of SNAP-25, a component of the neuronal SNARE complex. Inhibits SNARE-mediated vesicular neurotransmitter release at the neuromuscular junction when applied topically, reducing muscle contraction intensity and facial wrinkle depth. Marketed as a non-injectable alternative to botulinum toxin for cosmetic anti-aging applications.

§ I

At a glance

Route
Topical
Peptide length
8-AA
Primary target
SNARE
Route

Topical · Facial application · Twice daily

§ II

Mechanism

Edit ↗

Primary target — SNARE complex (SNAP-25 competitive binding site).

Pathway — Acetyl octapeptide-3 → SNARE complex disruption → Reduced vesicular fusion → Decreased acetylcholine release → Muscle relaxation.

Downstream effect — Transient reduction in neuromuscular signal transmission, decreased muscle contraction amplitude, wrinkle depth reduction.

Origin — Synthetic peptide derived from N-terminal fragment of SNAP-25 protein (synaptosomal-associated protein 25 kDa).

Feedback intact — N/A — topical cosmetic, no systemic endocrine axis.

§ III

Dosage

Protocols described in the cited literature; not medical advice.

Edit ↗
ParameterValue
Typical concentration2–10% in cosmetic formulations [lupin-2024][raikou-2017]Commercial products typically use 5–10%.
Application frequencyTwice daily (morning and evening) [lupin-2024]
Treatment duration20–60 days for visible effect [raikou-2017]Skin microtopography improvements measured at 20-day intervals.
Formulation typeOil-in-water emulsion, serum, cream
Application siteFacial skin — glabellar lines, crow's feet, forehead
Evidence basisRCT, in vitro skin penetration studies
Onset of effectVisible reduction in wrinkle depth by day 20–28 [raikou-2017]
§ III · b

Reconstitution

A pure mass-to-volume utility. Enter what you have in the vial; the atlas computes the volume per dose. No prescription information.

Inputs
mg
mL
mcg
The calculator does pure mass-to-volume math. It does not recommend a dose. Refer to SNAP-8's cited literature for protocol specifics.
Volumetric outputFig. C — reconstitution math
Volume per dose
0.100mL
10.0 units on a U-100 insulin syringe
Concentration
2500
mcg per mL
Doses per vial
20
at this dose
§ IV

Evidence

Edit ↗
Strength
0/100
theoretical

Not applicable — SNAP-8 is a cosmetic topical peptide for wrinkle reduction, not lipolysis.

OutcomeFinding
§ V

Adverse events

Severities follow the FDA / CTCAE convention.

Edit ↗
Cytotoxicitymild
Concentration-dependent antiproliferative effect observed in vitro; IC50 ~10 mg/mL (argireline, 6-AA analogue)
Skin irritationmild
Minimal; well-tolerated in clinical use
Peptide oxidationmild
Methionine residue susceptible to oxidation in formulation; may reduce efficacy [kluczyk-2021]
Systemic absorptionmild
Negligible; peptide remains in stratum corneum and epidermis [kraeling-2015]
Hypersensitivitymild
Rare; no widespread allergic reactions reported
Absolute contraindications
  • Known hypersensitivity to acetyl octapeptide-3 or formulation excipients
Relative contraindications
  • Active skin infections or open wounds at application site
  • Neuromuscular disorders (theoretical concern, no documented cases)
§ VI

Administration

Edit ↗
  1. 01
    Cleansing

    Wash face with gentle cleanser and pat dry. Remove makeup and surface oils to optimize peptide contact.

  2. 02
    Application

    Apply 1–2 drops or pea-sized amount of SNAP-8 serum or cream to target areas (forehead, glabellar lines, crow's feet). Gently massage until absorbed.

  3. 03
    Frequency

    Twice daily — morning and evening. Allow 2–3 minutes for absorption before applying additional skincare products.

  4. 04
    Layering

    Apply before heavier creams or occlusive moisturizers. Peptides penetrate best from water-based serums on clean skin.

  5. 05
    Storage

    Store at room temperature, away from direct sunlight. Refrigeration may extend shelf life of formulations containing unstable peptides.

  6. 06
    Duration

    Consistent use for 20–60 days required for visible wrinkle reduction. Effects are temporary and reverse upon discontinuation.

Appendix

Sources

17%

of 46 rendered claims carry a resolvable citation.

  1. [kluczyk-2021]
    Kluczyk 2021Argireline: Needle-Free Botox as Analytical Challenge.
    journal, 2021
  2. [kraeling-2015]
    Kraeling 2015In vitro skin penetration of acetyl hexapeptide-8 from a cosmetic formulation.
    journal, 2015
  3. [lupin-2024]
    Lupin 2024INDIVIDUAL ARTICLE: Real-World Clinical Experience With a Neuro-Peptide Serum in Combination With Botulinum Toxin Type-A Injections.
    journal, 2024
  4. [olsson-2024]
    Olsson 2024Public Interest in Acetyl Hexapeptide-8: Longitudinal Analysis.
    journal, 2024
  5. [raikou-2017]
    Raikou 2017The efficacy study of the combination of tripeptide-10-citrulline and acetyl hexapeptide-3. A prospective, randomized controlled study.
    journal, 2017
Plate composed 2026-04-27 · maturity human-reviewed · schema v1 · Contributors: peptidesdb-core · 38 fields uncited — open contributions