N-Acetyl Epitalon Amidate
also known as Epitalon, Epithalon, Ala-Glu-Asp-Gly, AEDG
Modified tetrapeptide (Ala-Glu-Asp-Gly) with N-acetyl and C-terminal amide caps for enhanced stability. Russian bioregulator developed by Khavinson et al. to activate telomerase in human somatic cells, induce telomere elongation, and overcome the Hayflick limit. In vitro studies show 10 additional passages in human fetal fibroblasts beyond natural senescence. Proposed mechanism: direct binding to promoter regions of telomerase, retinal genes, and RNA polymerase II to initiate transcription.
At a glance
SQ · Variable protocols
Primary target — DNA promoter regions (telomerase, RNA polymerase II, retinal genes).
Pathway — Peptide → DNA complementary binding → Gene transcription initiation → Telomerase catalytic subunit expression.
Downstream effect — Telomerase enzymatic activity induction, telomere elongation to early-passage length, extension of replicative lifespan in human somatic cells [khavinson-2003][khavinson-2004].
Origin — Synthetic tetrapeptide (Ala-Glu-Asp-Gly) derived from pineal extract bioregulator research; N-acetyl and C-amide modifications enhance plasma stability.
| Parameter | Value |
|---|---|
| Standard dose | No standardized human dosing in indexed literatureIn vitro protocols use direct culture addition; human clinical dosing protocols are in Russian-language literature outside PubMed scope. |
| Frequency | Not specified in candidate papers |
| Evidence basis | In vitro human cell culture [khavinson-2004][khavinson-2003] |
| Cell culture protocol | Addition to human fetal fibroblast culture induced telomerase activity and telomere elongation to early-passage length [khavinson-2004]Cells made 10 extra divisions (44 passages total vs 34 in control). |
| Duration | Chronic treatment in aging cultureSustained effect through late passages. |
| Modification stability | N-acetyl + C-amide caps enhance peptidase resistanceStandard strategy for tetrapeptide stabilization; specifics not quantified in candidates. |
Reconstitution
A pure mass-to-volume utility. Enter what you have in the vial; the atlas computes the volume per dose. No prescription information.
- — Active malignancy or history of cancer — telomerase reactivation may promote tumor cell immortalization
- — Individuals with hereditary cancer syndromes or high genetic cancer risk
- 01Route
Subcutaneous injection assumed based on peptide class; no specific protocol in candidate papers.
- 02Reconstitution
Standard bacteriostatic water for lyophilized peptides. Exact volume not specified in indexed literature.
- 03Storage
Lyophilized: -20 °C, desiccated. Reconstituted: refrigerate 2–8 °C. N-acetyl and C-amide modifications improve stability vs unprotected tetrapeptide.
- 04Clinical protocols
Human dosing schedules published in Russian-language clinical literature; not indexed in PubMed candidate set.
Sources
of 45 rendered claims carry a resolvable citation.
- [khavinson-2003]Khavinson 2003 — Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells
Bull Exp Biol Med, 2003 - [khavinson-2004]Khavinson 2004 — Peptide promotes overcoming of the division limit in human somatic cell.
journal, 2004 - [khavinson-2005]Khavinson 2005 — DNA double-helix binds regulatory peptides similarly to transcription factors.
journal, 2005